AngII对大鼠心肌梗死后TGF-β1表达的影响及对心室肌重构的作用.docVIP

AngII对大鼠心肌梗死的心肌 摘要: 目的：探讨ngiotensin II, AngII)对大鼠心肌梗死后转化生长因子-β1(transforming growth factor β1,TGF-β1 )表达的影响及对梗死心肌。方法：采用结扎冠状动脉前降支建立心肌梗死模型。术后48小时存活大鼠分为：假手术组、手术组、小剂量替米沙坦干预组、大剂量替米沙坦干预组。4 周后，取大鼠心脏左心室，采用免疫组化法、蛋白影迹（Western Blot）检测TGF-β1的表达变化。结果：手术组大鼠肌纤维呈空管状，心肌纤维肿胀、溶解断裂、坏死甚至融合成大片状, 可见大量心肌细胞核溶解消失，炎症细胞浸润等与假手术组比较,手术组大鼠TGF-β1表达显著增强( P 0. 05) ；替米沙坦TGF-β1表达减少( P 0. 05)。结论：AT1受体(angiotensin II type-1 receptor, AT1R)结合后可增加大鼠心肌梗死后TGF-β1的蛋白表达，AT1受体拮抗剂呈剂量依赖性抑制心肌梗死后TGF-β1的表达梗死心肌。 关键词: ； 心肌梗死； TGF-β；AT1Effect of telmisartan on TGF-β1 expression after Myocardial Infarction and Ventricular Remodeling in mice ABSTRACT: Objective: To investigate the effects of AngII on myocardium and expression of TGF-β1 after myocardial infarction( MI) in rats. Methods: The model of rats was made by ligating left ventricular branch. The sham operation group was made by the same procedure but not ligating.The rats were randomly divided into four groups after operation: A. sham operation group( Sham)， MI groups（MI）, MI+ telmisartan 10mg/（kg·d）, D, MI+telmisartan 20mg/（kg·d）). After 4 weeks, AngII and TGF-β1 was measured by immunohistochemical and western blot analysis. Results: Compared with the sham operation group, the expression of AngII and TGF-β1 of the MI group were increased significantly( P 0. 05)；compared with the MI group , expression of TGF-β1 was declined in the MI+ small telmisartan group and in the MI+ large telmisartan group( P 0. 05)；compared with the MI+ small telmisartan group , expression of TGF-β1 was declined in the MI+ large telmisartan group( P 0. 05).Conclusion: The expression of TGF-β1 increased by that AngII exerts its actions via interaction with the AT1R after myocardial infarction. AT1 recepter antagonist could decrease the expression of AngII和 TGF-β1 in myocardial and have a dose-dependent relationship . Key words: AngII, myocardial infarction, TGF-β1 , AT1 recepter antagonist 急性心肌梗死(AMI)是临床常见的急危重疾病，发病率逐年升高心肌梗死后心室重构与心肌梗死患者死亡率密切相关研究心室重构的机制寻求有效延缓或抑制心室重构的方法及降低心肌梗死后死亡率具有重要意义。