CO_2气腹对人卵巢癌SKOV3细胞裸鼠移植瘤CXCL12,CXCR4表达的影响.docVIP

,广东省科技计划资助项目2008B030301019 CO2气腹对人卵巢癌SKOV3细胞裸鼠移植瘤CXCL12、CXCR4表达的影响 张颖1） 刘莹2） 符爱珍2） 李元军2) 范炳娟2）△罗新1） （1暨南大学附属第一医院妇产科，广东广州，510630；2 广东医学院附属医院妇产科，广东湛江，524001） [摘 要] 目的 探讨二氧化碳 (CO2)气腹在相同压力条件下不同时间点对卵巢癌裸鼠移植瘤CXCL12、CXCR4表达的影响。方法 应用体外培养细胞注射法建立40只人卵巢癌SKOV3细胞裸鼠移植瘤动物模型，区组随机分组法将实验动物分为4组，每组10只：单纯麻醉组、开腹组0.5 h组、CO2气腹0.5 h组、CO2气腹1 h组, 成瘤后按上述分组处理后，取出瘤组织并采用PCR法检测各组中CXCL12，CXCR4 mRNA的表达，Western blot 法检测CXCL12，CXCR4蛋白表达。 结果 CO2气腹1 h组瘤体CXCL12 mRNA和蛋白表达显著高于其余3组（P0.05 ），而CXCR4 mRNA和蛋白表达差异无统计学意义(P 0.05 )。结论 CO2气腹1 h组可提高卵巢癌SKOV3细胞CXCL12的表达水平，但对CXCR4的表达水平无影响。 【关键词】气腹，卵巢癌，CXCL 12，CXCR4 【中图法分类号】 【文献标志码】A The effect of expression of CXCL12 and CXCR4 of CO2 pneumoperitoneum on nude mice xenografts from human ovarian cancer SKOV3 cells ZHANG-Ying1）LIU-Ying2 ）FUAI-Zhen2）LIYUAN-Jun2) FANBING-Juan2）LUO-Xin1） （1 Department of Obstetrics and Gynecology, The First Affiliated Hospital, Jinan University, Guangzhou 510630, China；2 Department of Obstetrics and Gynecology,the Affiliated Hospital of Guangdong Medical College,Zhanjiang 524001, China） [Abstract] Objective To investigate the impact of expression of CXCL12 and CXCR4 of carbon dioxide (CO2) pneumoperitoneum at different times in the same pressure on nude mice xenografts from human ovarian cancer SKOV3 cells. Methods 40 human ovarian cancer SKOV3 cells in nude mice xenograft tumor animal model were established by cultureds cell injection, then they were randomly divided into four groups (n=10) by block randomization method. There respectively were the simple anesthesia group, laparotomy group 0.5 h group, the CO2 pneumoperitoneum 0.5 h group. After the above treatment, the expression of CXCL12 mRNA and CXCR4 mRNA of tumor tissues were detected by RT-PCR, CXCL12 protein and CXCR4 protein were detected by Western blot. Results After the treatment, RT-PCR suggested The expression of CXCL12 mRNA in CO2 pneumoperitoneum 1h group was significantly higher than the other three groups, but CXCR4 mRNA no expression differences in each