CXCR4SDF-1对鼻咽癌细胞增殖与迁移能力的影响.docVIP

CXCR4/SDF-1对鼻咽癌细胞增殖与迁移能力的影响 邱际华1，刘求真1，陈丽2，焦锋3，姚开泰* (南方医科大学肿瘤研究所，广州 510515) 作者简介：邱际华(1981～ ），女，江西省赣州人，汉族,在读硕士研究生，主要从事肿瘤干细胞方面的研究，发表文章0篇，电话E-mail:qiujihua1015@163.com。 摘 要： 目的 观察鼻咽癌不同恶性程度的细胞中趋化因子受体 CXCR4 的表达, 检测 SDF- 1/CXCR4 反应轴对鼻咽癌细胞的增殖作用, SDF- 1 对 CXCR4 阳性肿瘤细胞的趋化作用,探讨 CXCR4 /SDF-1生物学轴对人鼻咽癌细胞增殖与迁移能力的影响. 方法 以鼻咽癌成瘤高转移细胞株5-8F及成瘤不转移细胞株6-10B为研究对象，采用Western Blot免疫印迹法检测鼻咽癌细胞株CXCR4蛋白的表达情况，SDF-1及CXCR4阻断剂AMD3100作用于两种细胞后 , MTT法检测细胞的增殖能力, 体外迁徙实验检测 SDF- 1/CXCR4 反应轴对鼻咽癌细胞的趋化作用。采用SPSS13.0 软件包进行 ANOVA 方差分析和 t 检验。结果 5-8F细胞株CXCR4蛋白的表达明显高于6-10B细胞株； SDF-1能明显增强5-8F细胞增殖与迁移能力， CXCR4阻断剂AMD3100作用后 ,5-8F细胞增殖与迁移能力明显降低；SDF-1对 6-10B细胞的迁移及增殖能力无明显影响。结论 CXCR4 /SDF-1生物学轴与鼻咽癌细胞株的增殖与迁移有一定的关系，AMD3100可明显抑制鼻咽癌细胞的增殖与迁移能力. 关键词：CXCR4； 鼻咽癌; 增殖，迁移。 Effect of CXCR4 /SDF-1 on proliferation and migration of Human Nasopharyngeal Carcinoma cell QIU Ji-hua1，LIU Qiu-hen1，YAO Kai-tai* (Institute of Cancer Research, Southern Medical University Guangzhou 510515) Abstract：Objective This study was aimed to evaluate the expression of chemokine receptors CXCR4 in NPC cells line and to determine the role of CXCR4 in tumor cell proliferation and migration. Methods The expression of CXCR4 protein in Human Nasopharyngeal Carcinoma cells was measured by Western Blot analysis . After the addition of SDF-1 and AMD3100, the changes of cell growth were observed by *通讯作者：姚开泰，男，院士， E-mail：ktyao@ 基金项目：广东省科技计划项目（2007B031515006） MTT, The changes of cell migration were measured by Transwell in vitro. Results Western Blot showed that 5-8F cells line, which tumorigenic and high metastatic ability ,express higher CXCR4 levels than 6-10B cell line, tumorigenic and lacking metastatic ability. the growth rate and the number of migration cell in 5-8F cells increased by SDF-1,and significantly decreased in vitro after the addition of AMD3100 ,while 6-10B cells were not in respond to SDF-1. Conclusion CXCR4/SDF-1 may play an important role in NPC cells proliferation and migration , AMD3100 can efficiently suppress its fun