quantitative methylation profiles for multiple tumor suppressor gene promoters in salivary gland tumors定量的多种肿瘤抑制基因启动子甲基化概要唾腺肿瘤.pdfVIP

Quantitative Methylation Profiles for Multiple Tumor Suppressor Gene Promoters in Salivary Gland Tumors 1 1 1 2 1 Megan L. Durr , Wojciech K. Mydlarz , Chunbo Shao , Marianna L. Zahurak , Alice Y. Chuang , 1 3 4 1,5 Mohammad O. Hoque , William H. Westra , Nanette J. Liegeois , Joseph A. Califano , David Sidransky1, Patrick K. Ha1,5* 1 Department of Otolaryngology-Head and Neck Surgery, Johns Hopkins Medical Institution, Baltimore, Maryland, United States of America, 2 Division of Oncology Biostatistics, Department of Oncology, Johns Hopkins Medical Institution, Baltimore, Maryland, United States of America, 3 Department of Pathology, Johns Hopkins Medical Institution, Baltimore, Maryland, United States of America, 4 Department of Oncology, Johns Hopkins Medical Institution, Baltimore, Maryland, United States of America, 5 Milton J. Dance, Jr. Head and Neck Center, Greater Baltimore Medical Center, Baltimore, Maryland, United States of America Abstract Background: Methylation profiling of tumor suppressor gene (TSGs) promoters is quickly becoming a powerful diagnostic tool for the early detection, prognosis, and even prediction of clinical response to treatment. Few studies address this in salivary gland tumors (SGTs); hence the promoter methylation profile of various TSGs was quantitatively assessed in primary SGT tissue to determine if tumor-specific alterations could be detected. Methodology: DNA isolated from 78 tumor and 17 normal parotid gland specimens was assayed for promoter methylation status of 19 TSGs by fluorescence-based, quantitative meth