quantitative pcr evaluation of cellular immune responses in kenyan children vaccinated with a candidate malaria vaccine定量pcr评价细胞免疫反应在肯尼亚的孩子接种疫苗候选疟疾疫苗.pdfVIP

Quantitative PCR Evaluation of Cellular Immune Responses in Kenyan Children Vaccinated with a Candidate Malaria Vaccine 1 2 1 2 1,2 Jedidah Mwacharo *, Susanna J. Dunachie , Oscar Kai , Adrian V. S. Hill , Philip Bejon , Helen A. Fletcher2 1 Centre for Geographical Medical Research, Kenya Medical Research Institute, Kilifi, Kenya, 2 The Jenner Institute, Nuffield Department of Clinical Medicine, University of Oxford, Churchill Hospital, Oxford, United Kingdom Abstract Background: The T-cell mediated immune response plays a central role in the control of malaria after natural infection or vaccination. There is increasing evidence that T-cell responses are heterogeneous and that both the quality of the immune response and the balance between pro-inflammatory and regulatory T-cells determines the outcome of an infection. As Malaria parasites have been shown to induce immunosuppressive responses to the parasite and non-related antigens this study examined T-cell mediated pro-inflammatory and regulatory immune responses induced by malaria vaccination in children in an endemic area to determine if these responses were associated with vaccine immunogenicity. Methods: Using real–time RT- PCR we profiled the expression of a panel of key markers of immunogenecity at different time points after vaccination with two viral vector vaccines expressing the malaria TRAP antigen (FP9-TRAP and MVA-TRAP) or following rabies vaccination as a control. Principal Findings: The vaccine induced modest levels of IFN-c mRNA one week after vaccination. There was also an increase in FoxP3 mRNA expression in both TRAP stimulated and media stimulated cells in the FFM ME-TRAP vaccine group; however, this may