quantitative proteomic analysis of the effect of fluoride on the acquired enamel pellicle定量蛋白质组学分析的氟化物在收购了搪瓷薄膜的影响.pdfVIP

Quantitative Proteomic Analysis of the Effect of Fluoride on the Acquired Enamel Pellicle 1 1,2 1 3 4 Walter L. Siqueira *, Meltem Bakkal , Yizhi Xiao , Jennifer N. Sutton , Fausto M. Mendes 1 Department of Biochemistry and School of Dentistry, Schulich School of Medicine and Dentistry, Western University, London, Onatrio, Canada, 2 Department of Pediatric Dentistry, School of Dentistry, Marmara University, Istanbul, Turkey, 3 Thermo Fisher Scientific, Cambridge, Massachusetts, United States of America, 4 Department of ˜ ˜ ˜ Pediatric Dentistry, Faculty of Dentistry, University of Sao Paulo, Sao Paulo, Sao Paulo, Brazil Abstract The acquired enamel pellicle (AEP) is a thin film formed by the selective adsorption of salivary proteins onto the enamel surface of teeth. The AEP forms a critical interface between the mineral phase of teeth (hydroxyapatite) and the oral microbial biofilm. This biofilm is the key feature responsible for the development of dental caries. Fluoride on enamel surface is well known to reduce caries by reducing the solubility of enamel to acid. Information on the effects of fluoride on AEP formation is limited. This study aimed to investigate the effects of fluoride treatment on hydroxyapatite on the subsequent formation of AEP. In addition, this study pioneered the use of label-free quantitative proteomics to better understand the composition of AEP proteins. Hydroxyapatite discs were randomly divided in 4 groups (n = 10 per group). Each disc was exposed to distilled water (control) or sodium fluoride solution (1, 2 or 5%) for 2 hours. Discs were then