quantitative proteomic analysis of the hfq-regulon in sinorhizobium meliloti 2011定量蛋白质组学分析的hfq-regulon sinorhizobium meliloti 2011.pdfVIP

Quantitative Proteomic Analysis of the Hfq-Regulon in Sinorhizobium meliloti 2011 1 ¨ 2¤a 3 3¤b 2¤a Patricio Sobrero , Jan-Philip Schluter , Ulrike Lanner , Andreas Schlosser , Anke Becker , Claudio Valverde1* ´ ´ ´ ´ 1 Laboratorio de Bioquımica, Microbiologıa e Interacciones Biologicas en el Suelo, Departamento de Ciencia y Tecnologıa, Universidad Nacional de Quilmes, Buenos Aires, Argentina, 2 Institute of Biology III, Faculty of Biology, University of Freiburg, Freiburg, Germany, 3 Core Facility Proteomics, Center for Biological Systems Analysis (ZBSA), Freiburg, Germany Abstract Riboregulation stands for RNA-based control of gene expression. In bacteria, small non-coding RNAs (sRNAs) are a major class of riboregulatory elements, most of which act at the post-transcriptional level by base-pairing target mRNA genes. The RNA chaperone Hfq facilitates antisense interactions between target mRNAs and regulatory sRNAs, thus influencing mRNA stability and/or translation rate. In the a-proteobacterium Sinorhizobium meliloti strain 2011, the identification and detection of multiple sRNAs genes and the broadly pleitropic phenotype associated to the absence of a functional Hfq protein both support the existence of riboregulatory circuits controlling gene expression to ensure the fitness of this bacterium in both free living and symbiotic conditions. In order to identify target mRNAs subject to Hfq-dependent riboregulation, we have compared the proteome of an hfq mutant and the wild type S. meliloti by quantitative proteomics following protein labelling with