rabies-specific antibodies measuring surrogates of protection against a fatal disease不防止致命疾病的抗体检测代理.pdfVIP

Review Rabies-Specific Antibodies: Measuring Surrogates of Protection against a Fatal Disease Susan M. Moore*, Cathleen A. Hanlon Rabies Laboratory, Kansas State Veterinary Diagnostic Laboratory, Kansas State University, Manhattan, Kansas, United States of America of the response, is necessary to obtain the measure or degree of Abstract: Antibodies play a central role in prophylaxis rabies immunity. against many infectious agents. While neutralization is a Initially, measurement of rabies virus neutralizing antibodies primary function of antibodies, the Fc- and complement- (RVNA) was performed in vivo using the mouse neutralization test dependent activities of these multifunctional proteins (MNT). Subsequently, the rapid fluorescent focus inhibition test may also be critical in their ability to provide protection (RFFIT) was established as the ‘‘gold standard’’ in vitro test [1]. against most viruses. Protection against viral pathogens in Methods for measuring rabies immunity vary with regard to the vivo is complex, and while virus neutralization—the ability of antibody to inactivate virus infectivity, often measured humoral component measured (i.e., the Ig subclass or functional in vitro—is important, it is often only a partial contributor activity) and performance characteristics (i.e., specificity or in protection. The rapid fluorescent focus inhibition test sensitivity), as well as th