rapid biocompatibility analysis of materials via in vivo fluorescence imaging of mouse models快速生物相容性材料通过体内荧光成像分析小鼠模型.pdfVIP

Rapid Biocompatibility Analysis of Materials via In Vivo Fluorescence Imaging of Mouse Models 1,2 1,2 3 4 4 Kaitlin M. Bratlie , Tram T. Dang , Stephen Lyle , Matthias Nahrendorf , Ralph Weissleder , Robert Langer1,2,5, Daniel G. Anderson2,5* 1 Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of America, 2 Department of Anesthesiology, Children’s Hospital Boston, Boston, Massachusetts, United States of America, 3 Department of Cancer Biology, University of Massachusetts Medical School, Worcester, Massachusetts, United States of America, 4 Center for Systems Biology, Massachusetts General Hospital, Boston, Massachusetts, United States of America, 5 David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of America Abstract Background: Many materials are unsuitable for medical use because of poor biocompatibility. Recently, advances in the high throughput synthesis of biomaterials has significantly increased the number of potential biomaterials, however current biocompatibility analysis methods are slow and require histological analysis. Methodology/Principal Findings: Here we develop rapid, non-invasive methods for in vivo quantification of the inflammatory response to implanted biomaterials. Materials were placed subcutaneously in an array format and monitored for host responses as per ISO 10993-6: 2001. Host cell activity in response to these materials was imaged kinetically, in vivo using fluorescent whole animal imaging. Data captured using whole animal imaging displayed similar temporal trends in cellular recruitment of phagocytes to the biomaterials