recombination drives genetic diversification of streptococcus dysgalactiae subspecies equisimilis in a region of streptococcal endemicity复合驱动基因多样化链球菌dysgalactiae亚种equisimilis链球菌风土性的一个地区.pdfVIP

Recombination Drives Genetic Diversification of Streptococcus dysgalactiae Subspecies equisimilis in a Region of Streptococcal Endemicity 1,2 3 4 5 2 David J. McMillan *, Santosh Y. Kaul , P. V. Bramhachari , Pierre R. Smeesters , Therese Vu , M. G. 3 4 1 Karmarkar , Melkote S. Shaila , Kadaba S. Sriprakash 1 Bacterial Pathogenesis Laboratory, Queensland Institute of Medical Research, Brisbane, Queensland, Australia, 2 Griffith Medical Research College, a joint collaboration between Griffith University and Queensland Institute of Medical Research, Brisbane, Queensland, Australia, 3 Department of Microbiology, KEM Hospital, Mumbai, India, 4 Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore, India, 5 Laboratoire de Genetique et Physiologie Bacterienne, Institut de Biologie et de Medecine Moleculaires, Faculte des Sciences, Universite Libre de Bruxelles, Brussels, Belgium Abstract Infection of the skin or throat by Streptococcus dysgalactiae subspecies equisimilis (SDSE) may result in a number of human diseases. To understand mechanisms that give rise to new genetic variants in this species, we used multi-locus sequence typing (MLST) to characterise relationships in the SDSE population from India, a country where streptococcal disease is endemic. The study revealed Indian SDSE isolates have sequence types (STs) predominantly different to those reported from other regions of the world. Emm-ST combinations in India are also largely unique. Split decomposition analysis, the presence of emm-types in unrelated clonal complexes, and analysis of phylogenetic trees based on c