regulation of slow and fast muscle myofibrillogenesis by wntβ-catenin and myostatin signaling慢速和快速调节肌肉myofibrillogenesis wntβ-catenin和肌肉生长抑制素的信号.pdfVIP

Regulation of Slow and Fast Muscle Myofibrillogenesis by Wnt/b-Catenin and Myostatin Signaling Jin-Ming Tee, Carina van Rooijen, Rick Boonen, Danica Zivkovic* Hubrecht Institute for Developmental Biology and Stem Cell Research and University Medical Center, Utrecht, The Netherlands Abstract Deviation from proper muscle development or homeostasis results in various myopathic conditions. Employing genetic as well as chemical intervention, we provide evidence that a tight regulation of Wnt/b-catenin signaling is essential for muscle fiber growth and maintenance. In zebrafish embryos, gain-of-Wnt/b-catenin function results in unscheduled muscle progenitor proliferation, leading to slow and fast muscle hypertrophy accompanied by fast muscle degeneration. The effects of Wnt/b-catenin signaling on fast muscle hypertrophy were rescued by misexpression of Myostatin or p21CIP/WAF, establishing an in vivo regulation of myofibrillogenesis by Wnt/b-catenin signaling and Myostatin. Epistatic analyses suggest a possible genetic interaction between Wnt/b-catenin and Myostatin in regulation of slow and fast twitch muscle myofibrillogenesis. Citation: Tee J-M, van Rooijen C, Boonen R, Zivkovic D (2009) Regulation of Slow and Fast Muscle Myofibrillogenesis by Wnt/ b-Catenin and Myostatin Signaling. PLoS ONE 4(6): e5880. doi:10.1371/journal.pone.0005880 Editor: Gianni Parise, McMaster University, Canada Received December 19, 2008; Accepted May 19, 2009; Published June 11, 2009 Copyright: 2009 Tee et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Funding: Work was funded by ALW Grant The fun