原创力文档reduced reactivation from dormancy but maintained lineage choice of human mesenchymal stem cells with donor age减少从休眠激活,但保持血统人类间充质干细胞供者年龄的选择.pdfVIP
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reduced reactivation from dormancy but maintained lineage choice of human mesenchymal stem cells with donor age减少从休眠激活,但保持血统人类间充质干细胞供者年龄的选择
Reduced Reactivation from Dormancy but Maintained
Lineage Choice of Human Mesenchymal Stem Cells with
Donor Age
1 2 2 1
Verena Dexheimer , Sebastian Mueller , Frank Braatz , Wiltrud Richter *
1 Research Center for Experimental Orthopedics, Orthopedic University Hospital Heidelberg, Heidelberg, Germany, 2 Department of Orthopedic Surgery, University of
Heidelberg, Heidelberg, Germany
Abstract
Mesenchymal stem cells (MSC) are promising for cell-based regeneration therapies but up to date it is still controversial
whether their function is maintained throughout ageing. Aim of this study was to address whether frequency, activation in
vitro, replicative function, and in vitro lineage choice of MSC is maintained throughout ageing to answer the question
whether MSC-based regeneration strategies should be restricted to younger individuals. MSC from bone marrow aspirates
of 28 donors (5–80 years) were characterized regarding colony-forming unit-fibroblast (CFU-F) numbers, single cell cloning
efficiency (SSCE), osteogenic, adipogenic and chondrogenic differentiation capacity in vitro. Alkaline phosphatase (ALP)
activity, mineralization, Oil Red O content, proteoglycan- and collagen type II deposition were quantified. While CFU-F
frequency was maintained, SSCE and early proliferation rate decreased significantly with advanced donor age. MSC with
higher proliferation rate before start of induction showed stronger osteogenic, adipogenic and chondrogenic
differentiation. MSC with high osteogenic capacity underwent better chondrogenesis and showed a trend to better
adipogenesis. Lineage choice was, however, unaltered with age. Conclusion: Ageing influenced activation fr
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