relationship between symptoms and gene expression induced by the infection of three strains of rice dwarf virus感染引起的症状和基因表达之间的关系三个水稻矮病毒株.pdfVIP

Relationship between Symptoms and Gene Expression Induced by the Infection of Three Strains of Rice dwarf virus 1,2 1 2 1 1 3 Kouji Satoh , Takumi Shimizu , Hiroaki Kondoh , Akihiro Hiraguri , Takahide Sasaya , Il-Ryong Choi , 1 2 Toshihiro Omura , Shoshi Kikuchi * 1 Research Team for Vector-borne Plant Pathogens, National Agricultural Research Center, Tsukuba, Ibaraki, Japan, 2 Division of Genome and Biodiversity Research, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki, Japan, 3 Plant Breeding, Genetics, and Biotechnology Division, International Rice Research Institute, Metro Manila, Philippines Abstract Background: Rice dwarf virus (RDV) is the causal agent of rice dwarf disease, which often results in severe yield losses of rice in East Asian countries. The disease symptoms are stunted growth, chlorotic specks on leaves, and delayed and incomplete panicle exsertion. Three RDV strains, O, D84, and S, were reported. RDV-S causes the most severe symptoms, whereas RDV-O causes the mildest. Twenty amino acid substitutions were found in 10 of 12 virus proteins among three RDV strains. Methodology/Principal Findings: We analyzed the gene expression of rice in response to infection with the three RDV strains using a 60-mer oligonucleotide microarray to examine the relationship between symptom severity and gene responses. The number of differentially expressed genes (DEGs) upon the infection of RDV-O, -D84, and -S was 1985, 3782, and 6726, respectively, showing a correlation between the number of DEGs and symptom severity. Many DEGs were related to defense, stress response, and development and morphogenesis processes. For d