release of sicam-1 in oocytes and in vitro fertilized human embryos释放sicam-1在人类卵母细胞和体外受精胚胎.pdfVIP

Release of sICAM-1 in Oocytes and In Vitro Fertilized Human Embryos 1. 2. 3 3 Monica Borgatti , Roberta Rizzo , Maria Beatrice Dal Canto , Daniela Fumagalli , Mario Mignini 3 3 2 2 1,4 Renzini , Rubens Fadini , Marina Stignani , Olavio Roberto Baricordi *, Roberto Gambari * 1 BioPharmaNet, Department of Biochemistry and Molecular Biology, University of Ferrara, Ferrara, Italy, 2 Department of Experimental and Diagnostic Medicine, Laboratory of Immunogenetics, University of Ferrara, Ferrara, Italy, 3 BIOGENESI Reproductive Medicine Centre, Istituti Clinici Zucchi, Monza, Italy, 4 Biotechnology Center, University of Ferrara, Ferrara, Italy Abstract Background: During the last years, several studies have reported the significant relationship between the production of soluble HLA-G molecules (sHLA-G) by 48–72 hours early embryos and an increased implantation rate in IVF protocols. As consequence, the detection of HLA-G modulation was suggested as a marker to identify the best embryos to be transferred. On the opposite, no suitable markers are available for the oocyte selection. Methodology/Principal Findings: The major finding of the present paper is that the release of ICAM-1 might be predictive of oocyte maturation. The results obtained are confirmed using three independent methodologies, such as ELISA, Bio-Plex assay and Western blotting. The sICAM-1 release is very high in immature oocytes, decrease in mature oocytes and become even lower in in vitro fertilized embryos. No significant differences were observed in the levels of sICAM-1 release betwee