原创力文档reliability at the lower limits of hiv-1 rna quantification in clinical samples a comparison of rt-pcr versus bdna assays可靠性下限的hiv - 1 rna在临床样本量化比较的rt - pcr和bdna化验.pdfVIP
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reliability at the lower limits of hiv-1 rna quantification in clinical samples a comparison of rt-pcr versus bdna assays可靠性下限的hiv - 1 rna在临床样本量化比较的rt - pcr和bdna化验
Reliability at the Lower Limits of HIV-1 RNA
Quantification in Clinical Samples: A Comparison of
RT-PCR versus bDNA Assays
1,2,3 2 3 1,2,3 1,2,3
Ronald J. Lubelchek *, Blake Max , Caroline J. Sandusky , Bala Hota , David E. Barker
1 Division of Infectious Diseases, John H. Stroger, Jr. Hospital of Cook County, Chicago, Illinois, United States of America, 2 Ruth M. Rothstein CORE Center, Chicago, Illinois,
United States of America, 3 Department of Internal Medicine, Rush University Medical Center, Chicago, Illinois, United States of America
Abstract
Introduction: To explore whether an assay change was responsible for an increasing proportion of patients with
undetectable HIV viral loads at our urban HIV clinic, we selected highly stable patients, examining their viral loads before
and after changing assays. We compared the proportion with detectable viremia during RT-PCR vs. bDNA periods.
Methodology/Principal Findings: We selected patients with $1 viral loads assessed during both RT-PCR and bDNA periods.
We included patients with stable CD4 counts, excluding patients with viral loads $1,000 copies/ml or any significant
changes in therapy. Out of 4500 clinic patients, 419 patients (1588 viral loads) were included. 39% of viral loads were
reported as detectable by RT-PCR vs. 5% reported as detectable by bDNA. The mean coefficient of variation was higher
before vs. after assay change. We found an odds’ ratio of 16.7 for having a viral load .75 copies/ml during the RT-PCR vs.
bDNA periods.
Discussion: These data support previous reports, suggesting that bDNA may more reliably discriminate between viral
suppression and low level
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