regulation of ccr5 expression in human placenta insights from a study of mother-to-child transmission of hiv in malawi监管ccr5的表达在人类胎盘从马拉维的母婴传播艾滋病毒的研究.pdfVIP

Regulation of CCR5 Expression in Human Placenta: Insights from a Study of Mother-to-Child Transmission of HIV in Malawi 1 1 2 1,3 1 Bonnie R. Joubert *, Nora Franceschini , Victor Mwapasa , Kari E. North , Steven R. Meshnick 1 Department of Epidemiology, Gillings School of Global Public Health, University of North Carolina, Chapel Hill, North Carolina, United States of America, 2 College of Medicine, University of Malawi, Blantyre, Malawi, 3 Carolina Center for Genome Sciences, University of North Carolina, Chapel Hill, North Carolina, United States of America Abstract Background: Human promoter polymorphisms in the chemokine co-receptor 5 gene (CCR5) have been noted for association with mother-to-child transmission of HIV (HIV MTCT) as well as reduced receptor expression in vitro, but have not been clearly associated with CCR5 expression in vivo. Placental expression of CCR5 may be influenced by such polymorphisms as well as other in vivo regulatory factors. Methodology/Principal Findings: We evaluated the associations between infant CCR5 polymorphisms, measures of maternal infection, and placental expression of CCR5 among mother-infant pairs in Blantyre, Malawi. RNA was extracted from placental tissue and used in multiplex real-time PCR to quantify gene expression. Through linear regression, we observed that CCR5-2554T (b = 20.67, 95% CI = 21.23, 20.11) and -2132T (b = 20.75, 95% CI = 20.131, 20.18) were significantly associated with reduced placental expression of CCR5. An incremental increase in CCR5 expression