原创力文档recombinant interferon-γ lentivirus co-infection inhibits adenovirus replication ex vivointerferon-γ重组慢病毒合并感染体外抑制腺病毒复制.pdfVIP
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recombinant interferon-γ lentivirus co-infection inhibits adenovirus replication ex vivointerferon-γ重组慢病毒合并感染体外抑制腺病毒复制
Recombinant Interferon-c Lentivirus Co-Infection Inhibits
Adenovirus Replication Ex Vivo
1 1 2 3 1 1 1
Ling Zhang , Sen Yin , Wanlong Tan , Dong Xiao , Yunceng Weng , Wenjing Wang , Tingting Li ,
3 4 1 5 1,6 1
Junwen Shi , Lifang Shuai , Hongwei Li , Jianhua Zhou , Jean-Pierre Allain , Chengyao Li *
1 Department of Transfusion Medicine, Southern Medical University, Guangzhou, China, 2 Department of Urology, Southern Medical University, Guangzhou, China,
3 Institute of Oncology, Southern Medical University, Guangzhou, China, 4 Guangzhou Military Centre of Disease Control, Guangzhou, China, 5 Laboratory of Lentiviruses
and Horse Diseases, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China, 6 Department of Hematology, University of Cambridge,
Cambridge, United Kingdom
Abstract
Recombinant interferon-c (IFNc) production in cultured lentivirus (LV) was explored for inhibition of target virus in cells co-
infected with adenovirus type 5 (Ad5). The ability of three different promoters of CMV, EF1a and Ubiquitin initiating the
enhanced green fluorescence protein (GFP) activities within lentiviruses was systematically assessed in various cell lines,
which showed that certain cell lines selected the most favorable promoter driving a high level of transgenic expression.
Recombinant IFNc lentivirus carrying CMV promoter (LV-CMV-IFNc) was generated to co-infect 293A cells with a viral
surrogate of recombinant GFP Ad5 in parallel with LV-CMV-GFP control. The best morphologic conditions were observed
from the t
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