relative roles of the cellular and humoral responses in the drosophila host defense against three gram-positive bacterial infections相对作用的细胞和体液反应果蝇宿主防御三个革兰氏阳性细菌感染.pdfVIP

Relative Roles of the Cellular and Humoral Responses in the Drosophila Host Defense against Three Gram-Positive Bacterial Infections 1.¤a 1. 1,2¤b 1,2 ´ 1 Nadine T. Nehme , Jessica Quintin , Ju Hyun Cho , Janice Lee , Marie-Celine Lafarge , Christine 1,2 1 Kocks , Dominique Ferrandon * ´ ´ 1 Equipe Fondation Recherche Medicale, UPR 9022 du Centre National de la Recherche Scientifique (CNRS), Institut de Biologie Moleculaire et Cellulaire du CNRS, ´ Universite de Strasbourg, Strasbourg, France, 2 Department of Pediatrics, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, United States of America Abstract Background: Two NF-kappaB signaling pathways, Toll and immune deficiency (imd), are required for survival to bacterial infections in Drosophila. In response to septic injury, these pathways mediate rapid transcriptional activation of distinct sets of effector molecules, including antimicrobial peptides, which are important components of a humoral defense response. However, it is less clear to what extent macrophage-like hemocytes contribute to host defense. Methodology/Principal Findings: In order to dissect the relative importance of humoral and cellular defenses after septic injury with three different Gram-positive bacteria (Micrococcus luteus, Enterococcus faecalis, Staphylococcus aureus), we used latex bead pre-injection to ablate macrophage function in flies wildtype or mutant for various Toll and imd pathway components. We found