rna-seq analyses generate comprehensive transcriptomic landscape and reveal complex transcript patterns in hepatocellular carcinomarna-seq生成全面的转录组分析景观在肝细胞癌和揭示复杂的转录模式.pdfVIP

RNA-Seq Analyses Generate Comprehensive Transcriptomic Landscape and Reveal Complex Transcript Patterns in Hepatocellular Carcinoma 1. 2,3. 1 1 2 2 2 1 Qichao Huang , Biaoyang Lin , Hanqiang Liu , Xi Ma , Fan Mo , Wei Yu , Lisha Li , Hongwei Li , Tian 4 5 5 1 1 Tian , Dong Wu , Feng Shen , Jinliang Xing *, Zhi-Nan Chen * 1 State Key Laboratory of Cancer Biology, Cell Engineering Research Center and Department of Cell Biology, Fourth Military Medical University, Xi’an, China, 2 Systems Biology Division, Zhejiang–California International Nanosystems Institute (ZCNI), Zhejiang University, Hangzhou, China, 3 Department of Urology, University of Washington, Seattle, Washington, United States of America, 4 Institute of Life Science and Biotechnology, Beijing Jiaotong University, Beijing, People’s Republic of China, 5 Department of Comprehensive Treatment, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai, People’s Republic of China Abstract RNA-seq is a powerful tool for comprehensive characterization of whole transcriptome at both gene and exon levels and with a unique ability of identifying novel splicing variants. To date, RNA-seq analysis of HBV-related hepatocellular carcinoma (HCC) has not been reported. In this study, we performed transcriptome analyses for 10 matched pairs of cancer and non-cancerous tissues from HCC patients on Solexa/Illumina GAII platform. On average, about 21.6 million sequencing reads and 10.6 million aligned reads were obtained for samples sequenced on each lane, which was able to identify .50% of all the annotated genes for each sample. Furthermore, we identified