retinal ganglion cell loss is accompanied by antibody depositions and increased levels of microglia after immunization with retinal antigens视网膜神经节细胞损失是伴随着抗体口供和水平的提高与视网膜小胶质细胞在免疫抗原.pdfVIP

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retinal ganglion cell loss is accompanied by antibody depositions and increased levels of microglia after immunization with retinal antigens视网膜神经节细胞损失是伴随着抗体口供和水平的提高与视网膜小胶质细胞在免疫抗原.pdf

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retinal ganglion cell loss is accompanied by antibody depositions and increased levels of microglia after immunization with retinal antigens视网膜神经节细胞损失是伴随着抗体口供和水平的提高与视网膜小胶质细胞在免疫抗原

Retinal Ganglion Cell Loss Is Accompanied by Antibody Depositions and Increased Levels of Microglia after Immunization with Retinal Antigens 1 2 2 1 2 Stephanie C. Joachim *, Oliver W. Gramlich , Panagiotis Laspas , Heiko Schmid , Sabine Beck , 3 1 2 2 Harald D. von Pein , H. Burkhard Dick , Norbert Pfeiffer , Franz H. Grus 1 Experimental Eye Research Institute, Ruhr University Eye Hospital, Bochum, Germany, 2 Experimental Ophthalmology, Department of Ophthalmology, University Medical Center, Johannes Gutenberg University, Mainz, Germany, 3 Department of Neuropathology, University Medical Center, Johannes Gutenberg University, Mainz, Germany Abstract Background: Antibodies against retinal and optic nerve antigens are detectable in glaucoma patients. Recent studies using a model of experimental autoimmune glaucoma demonstrated that immunization with certain ocular antigens causes an immun-mediated retinal ganglion cell loss in rats. Methodology/Principal Findings: Rats immunized with a retinal ganglion cell layer homogenate (RGA) had a reduced retinal ganglion cell density on retinal flatmounts (p = 0.007) and a lower number of Brn3+retinal ganglion cells (p = 0.0001) after six weeks. The autoreactive antibody development against retina and optic nerve was examined throughout the study. The levels of autoreactive antibodies continuously increased up to 6 weeks (retina: p = 0.004; optic nerve: p = 0.000003). Additionally, antibody deposits were detected in the retina (p = 0.02). After 6 weeks a reactive gliosis (GFAP density: RGA: 174.7641.9; CO: 137.6 636.8, p = 0.0006; %GFAP+ area: RGA: 8.5 63.4; CO: