rna-seq reveals an integrated immune response in nucleated erythrocytesrna-seq揭示了一个集成的有核红细胞的免疫反应.pdfVIP

RNA-Seq Reveals an Integrated Immune Response in Nucleated Erythrocytes 1 1 2 2 ˜ 2 1 Davinia Morera , Nerea Roher , Laia Ribas , Joan Carles Balasch , Carmen Donate , Agnes Callol , ˜ 1 3 4 4 1 Sebastian Boltana , Steven Roberts , Giles Goetz , Frederick W. Goetz , Simon A. MacKenzie * ` 1 Institute of Biotechnology and Biomedicine, Universitat Autonoma de Barcelona, Barcelona, Spain, 2 Unitat de Fisiologia Animal, Departament de Biologia Cel.lular, ` ` Fisiologia i Immunologia, Facultat de Ciencies, Universitat Autonoma de Barcelona, Bellaterra (Barcelona), Spain, 3 School of Aquatic and Fishery Sciences, University of Washington, Seattle, Washington, United States of America, 4 School of Freshwater Sciences, University of Wisconsin-Milwaukee, Milwaukee, Wisconsin, United States of America Abstract Background: Throughout the primary literature and within textbooks, the erythrocyte has been tacitly accepted to have maintained a unique physiological role; namely gas transport and exchange. In non-mammalian vertebrates, nucleated erythrocytes are present in circulation throughout the life cycle and a fragmented series of observations in mammals support a potential role in non-respiratory biological processes. We hypothesised that nucleated erythrocytes could actively participate via ligand-induced transcriptional re-programming in the immune response. Methodology/Principal Findings: Nucleated erythrocytes from both fish and birds express and regulate specific p