role for a-type lamins in herpesviral dna targeting and heterochromatin modulation角色a类型核纤层蛋白在herpesviral dna靶向和异染色质调制.pdfVIP

Role for A-Type Lamins in Herpesviral DNA Targeting and Heterochromatin Modulation Lindsey Silva, Anna Cliffe, Lynne Chang, David M. Knipe* Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts, United States of America Abstract Posttranslational modification of histones is known to regulate chromatin structure and transcriptional activity, and the nuclear lamina is thought to serve as a site for heterochromatin maintenance and transcriptional silencing. In this report, we show that the nuclear lamina can also play a role in the downregulation of heterochromatin and in gene activation. Herpes simplex virus DNA initiates replication in replication compartments near the inner edge of the nucleus, and histones are excluded from these structures. To define the role of nuclear lamins in HSV replication, we examined HSV infection in wild- type and A-type lamin–deficient (Lmna2/ 2) murine embryonic fibroblasts (MEFs). In Lmna2/ 2 cells, viral replication compartments are reduced in size and fail to target to the nuclear periphery, as observed in WT cells. Chromatin immunoprecipitation and immunofluorescence studies demonstrate that HSV DNA is associated with increased heterochromatin in Lmna2/ 2 MEFs. These results argue for a functional role for A-type lamins as viral gene expression, DNA replication, and growth are reduced in Lmna2/ 2 MEFs, with the greatest effect on viral replication at low multiplicity of infection. Thus, lamin A/C is required for targeting of the viral genome and the reduction of heterochromatin on viral promoters during lytic infection. The nuclear lamina can serve as a molecular scaffold for DNA genomes and the protein complexes that regulate both euchromatin and he