reveille8 and pseudo-reponse regulator5 form a negative feedback loop within the arabidopsis circadian clockreveille8和pseudo-reponse regulator5形成负面反馈循环在拟南芥生物钟.pdfVIP

REVEILLE8 and PSEUDO-REPONSE REGULATOR5 Form a Negative Feedback Loop within the Arabidopsis Circadian Clock 1 1. 1. 1 1 Reetika Rawat , Nozomu Takahashi , Polly Yingshan Hsu , Matthew A. Jones , Jacob Schwartz , 2 2 1 Michelle R. Salemi , Brett S. Phinney , Stacey L. Harmer * 1 Department of Plant Biology, College of Biological Sciences, University of California Davis, Davis, California, United States of America, 2 Genome Center, Proteomics Core, Genome and Biomedical Sciences Facility, University of California Davis, Davis, California, United States of America Abstract Circadian rhythms provide organisms with an adaptive advantage, allowing them to regulate physiological and developmental events so that they occur at the most appropriate time of day. In plants, as in other eukaryotes, multiple transcriptional feedback loops are central to clock function. In one such feedback loop, the Myb-like transcription factors CCA1 and LHY directly repress expression of the pseudoresponse regulator TOC1 by binding to an evening element (EE) in the TOC1 promoter. Another key regulatory circuit involves CCA1 and LHY and the TOC1 homologs PRR5, PRR7, and PRR9. Purification of EE–binding proteins from plant extracts followed by mass spectrometry led to the identification of RVE8, a homolog of CCA1 and LHY. Similar to these well-known clock genes, expression of RVE8 is circadian-regulated with a dawn phase of expression, and RVE8 binds specifically to the EE. However, whereas cca1 and lhy mutants have short period phenotypes and overexpression of either gene causes arrhythmia, rve8 mutants have