reverse engineering of bacterial chemotaxis pathway via frequency domain analysis逆向工程的细菌趋化性途径通过频域分析.pdfVIP

Reverse Engineering of Bacterial Chemotaxis Pathway via Frequency Domain Analysis 1 2 1 1 Junjie Luo , Jun Wang , Ting Martin Ma , Zhirong Sun * 1 Ministry of Education Key Laboratory of Bioinformatics, Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing, People’s Republic of China, 2 Department of Computer Science, Tsinghua University, Beijing, People’s Republic of China Abstract Chemotaxis is defined as a behavior involving organisms sensing attractants or repellents and leading towards or away from them. Therefore, it is possible to reengineer chemotaxis network to control the movement of bacteria to our advantage. Understanding the design principles of chemotaxis pathway is a prerequisite and an important topic in synthetic biology. Here, we provide guidelines for chemotaxis pathway design by employing control theory and reverse engineering concept on pathway dynamic design. We first analyzed the mathematical models for two most important kinds of E. coli chemotaxis pathway—adaptive and non-adaptive pathways, and concluded that the control units of the pathway de facto function as a band-pass filter and a low-pass filter, respectively, by abstracting the frequency response properties of the pathways. The advantage of the band-pass filter is established, and we demonstrate how to tune the three key parameters of it—A (max amplification), v1 (down cut-off frequency) and v2 (up cut-off frequency) to optimize the chemotactic effect. Finally, we hypothesized a similar but simpler version of the dynamic pathway model based on the principles discovered and show that it leads to similar properties with native E. coli chemotactic behaviors. Our study provides an example of simulating and designing biological dynamics in silico and indicates how to make