review of two decades of cholera diagnostics – how far have we really come回顾二十年的霍乱诊断u2014u2014我们真的走了多远.pdfVIP

Review of Two Decades of Cholera Diagnostics – How Far Have We Really Come? 1 2 2 3 Michal H. Dick *, Martine Guillerm , Francis Moussy , Claire-Lise Chaignat 1 Medical School, Australian National University, Canberra, Australia, 2 United Nations International Children’s Emergency Fund/United Nations Development Program/ World Bank/World Health Organisation Special Programme for Research and Training in Tropical Diseases, World Health Organization, Geneva, Switzerland, 3 Global Task Force on Cholera Control, Department of Public Health and Environment, Health Security and Environment, World Health Organization, Geneva, Switzerland Abstract Background: Cholera, an ancient scourge, continues to inflict high rates of mortality today. The rising incidence of epidemics in areas of poor sanitation and crowding highlight the need for better epidemic prevention and early response. Such interventions require the availability of rapid and accurate diagnostic techniques to trigger timely response and mitigate the scale of the outbreak. The current gold standard of bacterial culture is inadequate for rapid diagnosis, highlighting the overarching neglect of field diagnostic needs. This paper was written to support the World Health Organisation’s Global Task Force on Cholera Control mandated Cholera and diarrhoeal disease laboratory Network (CholdiNet) in devising a protocol for the validation of Rapid Diagnostic Tests (RDTs) for Vibrio cholerae. The status of diagnostic tools for Vibrio cholerae is assessed, describing products that have been commercialised over the last two decades and discussing their peer-reviewed evaluation. Method: R