revisiting host preference in the mycobacterium tuberculosis complex experimental infection shows m. tuberculosis h37rv to be avirulent in cattle主机优先考虑结核分枝杆菌感染结核分枝杆菌复杂的实验显示h37rv无毒的牛.pdfVIP

Revisiting Host Preference in the Mycobacterium tuberculosis Complex: Experimental Infection Shows M. tuberculosis H37Rv to Be Avirulent in Cattle 1 1 1¤ 1 1 Adam O. Whelan , Michael Coad , Paul J. Cockle , Glyn Hewinson , Martin Vordermeier , Stephen V. Gordon1,2,3,4,5* 1TB Research Group, Veterinary Laboratories Agency Weybridge, New Haw, United Kingdom, 2 School of Agriculture, Food Science and Veterinary Medicine, University College Dublin, Dublin, Ireland, 3 School of Medicine and Medical Science, University College Dublin, Dublin, Ireland, 4 School of Biomolecular and Biomedical Science, College of Life Sciences, University College Dublin, Dublin, Ireland, 5 Conway Institute of Biomolecular and Biomedical Research, University College Dublin, Dublin, Ireland Abstract Experiments in the late 19th century sought to define the host specificity of the causative agents of tuberculosis in mammals. Mycobacterium tuberculosis, the human tubercle bacillus, was independently shown by Smith, Koch, and von Behring to be avirulent in cattle. This finding was erroneously used by Koch to argue the converse, namely that Mycobacterium bovis, the agent of bovine tuberculosis, was avirulent for man, a view that was subsequently discredited. However, reports in the literature of M. tuberculosis isolation from cattle with tuberculoid lesions suggests that the virulence of M. tuberculosis for cattle needs to be readdressed. We used an experimental bovine infection model to test the virulence of well-characterized strains of M. tuberculosis and M. bovis in cattle, choosing the genome-sequenced strains M. tuberculosis H37Rv and M. bovis 2122/97. Cattle were infected with approximatel