replication timing of human telomeres is chromosome arm–specific, influenced by subtelomeric structures and connected to nuclear localization人类端粒是染色体arm-specific复制时间,受到subtelomeric结构和连接到核本地化.pdfVIP

Replication Timing of Human Telomeres Is Chromosome Arm–Specific, Influenced by Subtelomeric Structures and Connected to Nuclear Localization 1 2 3 1 Nausica Arnoult , Caroline Schluth-Bolard , Anne Letessier , Irena Drascovic , Rachida 1 4,5 4¤ 2 2 Bouarich-Bourimi , Judith Campisi , Sahn-ho Kim , Amina Boussouar , Alexandre Ottaviani , ´ ´ 2 2 ˜ 1 Frederique Magdinier , Eric Gilson , Arturo Londono-Vallejo * 1Telomeres and Cancer Laboratory, Institut Curie, CNRS, UPMC University Paris 06, Paris, France, 2 Epigenetics and Telomere Regulation, CNRS ENS UCBL IFR128, Ecole ´ Normale Superieure de Lyon, Lyon, France, 3 Functional Organization and Plasticity of Mammalian Genomes, Institut Curie, UPMC University Paris 06, Paris, France, 4 Lawrence Berkeley Laboratory, Berkeley, California, United States of America, 5 Buck Institute for Age Research, Novato, California, United States of America Abstract The mechanisms governing telomere replication in humans are still poorly understood. To fill this gap, we investigated the timing of replication of single telomeres in human cells. Using in situ hybridization techniques, we have found that specific telomeres have preferential time windows for replication during the S-phase and that these intervals do not depend upon telomere length and are largely conserved between homologous chromosomes and between individuals, even in the presence of large subtelomeric segmental polymorphisms. Importantly, we show that one copy of the 3.3 kb macrosatellite