regulation of microrna-155 in atherosclerotic inflammatory responses by targeting map3k10调节微rna - 155在针对map3k10动脉粥样硬化炎症反应.pdfVIP

Regulation of MicroRNA-155 in Atherosclerotic Inflammatory Responses by Targeting MAP3K10 1 1 1 3 4 1 2 Jianhua Zhu , Ting Chen , Lin Yang , Zhoubin Li , Mei Mei Wong , Xiaoye Zheng , Xiaoping Pan , Li Zhang1*, Hui Yan1 1 Department of Cardiology, First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, People’s Republic of China, 2 Department of Infectious Disease, First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, People’s Republic of China, 3 Department of Cardiothoracic Surgery, First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, People’s Republic of China, 4 Cardiovascular Division, School of Medicine, King’s College London, London, United Kingdom Abstract Aims: Accumulating evidence suggest that numerous microRNAs (miRNAs) play important roles in cell proliferation, apoptosis, and differentiation, as well as various diseases that accompany inflammatory responses. Inflammation is known to be a major contributor to atherogenesis. Previous studies provide promising evidence in support of the role of miRNAs in cardiovascular disease. However, mechanistic data on these small molecules in atherosclerosis (AS) are still missing. The present study aims to investigate the potential role of miRNAs in AS. Methods and Results: The miRNA transcriptase was verified by TaqMan real-time polymerase chain reaction assay. Thoracic aorta samples were obtained from Apolipoprotein E knockout mice, and plasma samples were from coronary artery disease (CAD) patients. The results showed that the miR-155 level was the most significantly elevated both in AS mice and CAD patients relative to the normal control. The functional role of miR-155 in the atheros