原创力文档reduced expression of transcription factor ap-2α is associated with gastric adenocarcinoma prognosis转录因子的表达减少ap-2α与胃腺癌预后相关.pdfVIP
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reduced expression of transcription factor ap-2α is associated with gastric adenocarcinoma prognosis转录因子的表达减少ap-2α与胃腺癌预后相关
Reduced Expression of Transcription Factor AP-2a Is
Associated with Gastric Adenocarcinoma Prognosis
1,2. 1. 1 1,3 1 1 1
Wei Wang , Lin Lv , Ke Pan , Yu Zhang , Jing-jing Zhao , Ju-gao Chen , Yi-bing Chen , Yong-qiang
1 1 1 1 1,2 1
Li , Qi-jin Wang , Jia He , Shi-ping Chen , Zhi-wei Zhou *, Jian-chuan Xia *
1 State Key Laboratory of Oncology in South China and Department of Experimental Research, Cancer Center, Sun Yat-sen University, Guangzhou, People’s Republic of
China, 2 Department of Gastric and Pancreatic Surgery, Cancer Center, Sun Yat-sen University, Guangzhou, People’s Republic of China, 3 Department of Pathology, Cancer
Center, Sun Yat-sen University, Guangzhou, People’s Republic of China
Abstract
Background: This study aims to investigate the expression and prognostic significance of activator protein 2a (AP-2a) in
gastric adenocarcinoma.
Methodology/Principal Findings: AP-2a expression was analyzed using real-time quantitative PCR (RT-qPCR), western
blotting, and immunohistochemical staining methods on tissue samples from a consecutive series of 481 gastric
adenocarcinoma patients who underwent resections between 2003 and 2006. The relationship between AP-2a expression,
clinicopathological factors, and patient survival was investigated. RT- qPCR results showed that the expression of AP-2a
mRNA was reduced in tumor tissue samples, compared with expression in matched adjacent non-tumor tissue samples
(P = 0.009); this finding was confirmed by western blotting analysis (P = 0.012). Immunohistochemical staining
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