原创力文档reduced expression of inflammatory genes in deceased donor kidneys undergoing pulsatile pump preservation减少炎症基因的表达在死者供体肾脏接受脉动的泵保护.pdfVIP
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reduced expression of inflammatory genes in deceased donor kidneys undergoing pulsatile pump preservation减少炎症基因的表达在死者供体肾脏接受脉动的泵保护
Reduced Expression of Inflammatory Genes in Deceased
Donor Kidneys Undergoing Pulsatile Pump Preservation
1 2 3 1 1 4
Valeria R. Mas *, Kellie J. Archer , Catherine I. Dumur , Mariano J. Scian , Jihee L. Suh , Anne L. King ,
5 5 4 5 5
Megan E. Wardius , Julie A. Straub , Marc P. Posner , Kenneth Brayman , Daniel G. Maluf
1Translational Genomics Transplant Laboratory, University of Virginia, Charlottesville, Virginia, United States of America, 2 Department of Biostatistics, Virginia
Commonwealth University, Richmond, Virginia, United States of America, 3 Department of Pathology, Virginia Commonwealth University, Richmond, Virginia, United
States of America, 4 Transplant Division, Department of Surgery, Virginia Commonwealth University, Richmond, Virginia, United States of America, 5 Transplant Division,
Department of Surgery, University of Virginia, Charlottesville, Virginia, United States of America
Abstract
Background: The use of expanded criteria donor kidneys (ECD) had been associated with worse outcomes. Whole gene
expression of pre-implantation allograft biopsies from deceased donor kidneys (DDKs) was evaluated to compare the effect
of pulsatile pump preservation (PPP) vs. cold storage preservation (CSP) on standard and ECD kidneys.
Methodology/Principal Findings: 99 pre-implantation DDK biopsies were studied using gene expression with GeneChips.
Kidneys transplant recipients were followed post transplantation for 35.8 months (range = 24–62). The PPP group included
60 biopsies (cold ischemia time (CIT) = 1,367+/ 2509 minutes) and the CSP group included 39 biopsies (CIT = 1,022+/
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