regulation of glioblastoma progression by cord blood stem cells is mediated by downregulation of cyclin d1监管的胶质母细胞瘤进展脐带血干细胞细胞周期蛋白d1的差别是由对这些.pdfVIP

Regulation of Glioblastoma Progression by Cord Blood Stem Cells Is Mediated by Downregulation of Cyclin D1 1 1 2 1 Kiran Kumar Velpula , Venkata Ramesh Dasari , Andrew J. Tsung , Christopher S. Gondi , Jeffrey D. 2 1 1,2 Klopfenstein , Sanjeeva Mohanam , Jasti S. Rao * 1 Department of Cancer Biology and Pharmacology, University of Illinois College of Medicine at Peoria, Peoria, Illinois, United States of America, 2 Department of Neurosurgery, University of Illinois College of Medicine at Peoria, Peoria, Illinois, United States of America Abstract Background: The normal progression of the cell cycle requires sequential expression of cyclins. Rapid induction of cyclin D1 and its associated binding with cyclin-dependent kinases, in the presence or absence of mitogenic signals, often is considered a rate-limiting step during cell cycle progression through the G1 phase. Methodology/Principal Findings: In the present study, human umbilical cord blood stem cells (hUCBSC) in co-cultures with glioblastoma cells (U251 and 5310) not only induced G -G phase arrest, but also reduced the number of cells at S and G -M 0 1 2 phases of cell cycle. Cell cycle regulatory proteins showed decreased expression levels upon treatment with hUCBSC as revealed by Western and FACS analyses. Inhibition of cyclin D1 activity by hUCBSC treatment is sufficient to abolish the expression levels of Cdk 4, Cdk 6, cyclin B1, b-Catenin levels. Our immuno precipit