reduced body weight and increased energy expenditure in transgenic mice over-expressing soluble leptin receptor减少体重和增加能量消耗在转基因小鼠过度表达可溶性瘦素受体.pdfVIP

Reduced Body Weight and Increased Energy Expenditure in Transgenic Mice Over-Expressing Soluble Leptin Receptor 1 1 2 2 2 1 Phing-How Lou , Guoqing Yang , Lu Huang , Yunxia Cui , Tiffany Pourbahrami , George K. Radda , Cai 2 ¤ 1,3 Li * , Weiping Han * 1 Laboratory of Metabolic Medicine, Singapore Bioimaging Consortium, Agency for Science, Technology and Research (A*STAR), Singapore, Singapore, 2 Department of Physiology, Touchstone Center for Diabetes Research, The University of Texas Southwestern Medical Center, Dallas, Texas, United States of America, 3 Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore Abstract Background: Soluble leptin receptor (OBRe), one of several leptin receptor isoforms, is the only bona fide leptin binding protein in plasma. Our earlier studies demonstrated that OBRe modulates leptin levels in circulation. Both clinical and in vitro studies have shown that OBRe expression is inversely correlated to body weight and leptin levels. However, it is not clear whether OBRe plays an active role, either in collaboration with leptin or independently, in the maintenance of body weight. Methodology/Principal Findings: To investigate the function of OBRe in the regulation of energy homeostasis, we generated transgenic mice that express OBRe under the control of human serum amyloid P (hSAP) component gene promoter. The transgene led to approximately doubling of OBRe in circulation in the transgenic mice than in wild type control mice. Transgenic mice exhibited lower body weight at 4 weeks of age, and slower rate of weight gain when compared with con