rapid dopaminergic modulation of the fish hypothalamic transcriptome and proteome快速的多巴胺能调制鱼下丘脑转录组和蛋白质组.pdfVIP

Rapid Dopaminergic Modulation of the Fish Hypothalamic Transcriptome and Proteome 1 2 2 1 Jason T. Popesku , Christopher J. Martyniuk , Nancy D. Denslow , Vance L. Trudeau * 1 Centre for Advanced Research in Environmental Genomics, Department of Biology, University of Ottawa, Ottawa, Ontario, Canada, 2 Department of Physiological Sciences and Center for Environmental and Human Toxicology, University of Florida, Gainesville, Florida, United States of America Abstract Background: Dopamine (DA) is a major neurotransmitter playing an important role in the regulation of vertebrate reproduction. We developed a novel method for the comparison of transcriptomic and proteomic data obtained from in vivo experiments designed to study the neuroendocrine actions of DA. Methods and Findings: Female goldfish were injected (i.p.) with DA agonists (D1-specific; SKF 38393, or D2-specific; LY 171555) and sacrificed after 5 h. Serum LH levels were reduced by 57% and 75% by SKF 38393 and LY 171555, respectively, indicating that the treatments produced physiologically relevant responses in vivo. Bioinformatic strategies and a ray-finned fish database were established for microarray and iTRAQ proteomic analysis of the hypothalamus, revealing a total of 3088 mRNAs and 42 proteins as being differentially regulated by the treatments. Twenty one proteins and mRNAs corresponding to these proteins appeared on both lists. Many of the mRNAs and proteins affected by the treatments were grouped into the Gene Ontology categorizations of protein complex, signal transduction, response to stimulus, and regulation of cellular processes. There was a 57% and 14% directional agreement between the differentially-regulated mRNAs a