rapid etiological classification of meningitis by nmr spectroscopy based on metabolite profiles and host response快速病因分类脑膜炎的核磁共振光谱学基于代谢物概要文件和宿主反应.pdfVIP

Rapid Etiological Classification of Meningitis by NMR Spectroscopy Based on Metabolite Profiles and Host Response 1,2¤ 3 ¨ 4 1,5 6 Uwe Himmelreich *, Richard Malik , Till Kuhn , Heide-Marie Daniel , Ray L. Somorjai , Brion Dolenko6, Tania C. Sorrell1 1 Centre for Infectious Diseases and Microbiology and Westmead Millennium Institute and NHMRC Centre of Clinical Research Excellence in Infections and Bioethics in Haematological Malignancies, The University of Sydney, Westmead, Australia, 2 Institute for Magnetic Resonance Research, The University of Sydney, St Leonards, New South Wales, Australia, 3 Post Graduate Foundation in Veterinary Science and Faculty of Veterinarian Sciences, The University of Sydney, St Leonards, New South Wales, ¨ ´ ` ´ Australia, 4 Bruker Biospin, Fallanden, Switzerland, 5 Unite de Microbiologie, Mycotheque de l’Universite catholique de Louvain, Louvain-la-Neuve, Belgium, 6 Institute for Biodiagnostics, National Research Council of Canada, Winnipeg, Manitoba, Canada Abstract Bacterial meningitis is an acute disease with high mortality that is reduced by early treatment. Identification of the causative microorganism by culture is sensitive but slow. Large volumes of cerebrospinal fluid (CSF) are required to maximise sensitivity and establish a provisional diagnosis. We have utilised nuclear magnetic resonance (NMR) spectroscopy to rapidly characterise the biochemical profile of CSF from normal rats and animals with pneumococcal or cryptococcal meningitis. Use of a miniaturised capillary NMR system overcame limitations caused by small CSF volumes and low metabolite concentrations. The analysis o