reverse taxonomy for elucidating diversity of insect-associated nematodes a case study with termites反向分类法阐明insect-associated线虫多样性与白蚁的案例研究.pdfVIP

Reverse Taxonomy for Elucidating Diversity of Insect- Associated Nematodes: A Case Study with Termites 1,2 1 1 2 3 Natsumi Kanzaki *, Robin M. Giblin-Davis , Rudolf H. Scheffrahn , Hisatomo Taki , Alejandro Esquivel , Kerrie A. Davies4, E. Allen Herre5 1 Fort Lauderdale Research and Education Center, University of Florida/IFAS, Davie, Florida, United States of America, 2 Forestry and Forest Products Research Institute, Tsukuba, Ibaraki, Japan, 3 Universidad Nacional, Escuela de Ciencias Agrarias, Laboratorio de Nematologia, Heredia, Costa Rica, 4 Centre for Evolutionary Biology and Biodiversity, School of Agriculture, Food and Wine, The University of Adelaide, Waite Campus, Glen Osmond, South Australia, Australia, 5 Smithsonian Tropical Research Institute, Balboa, Ancon, Republic of Panama Abstract Background: The molecular operational taxonomic unit (MOTU) has recently been applied to microbial and microscopic animal biodiversity surveys. However, in many cases, some of the MOTUs cannot be definitively tied to any of the taxonomic groups in current databases. To surmount these limitations, the concept of ‘‘reverse taxonomy’’ has been proposed, i.e. to primarily list the MOTUs with morphological information, and then identify and/or describe them at genus/species level using subsamples or by re-isolating the target organisms. Nevertheless, the application of ‘‘reverse taxonomy’’ has not been sufficiently evaluated. Therefore, the practical applicability of ‘‘reverse taxonomy’’ is tested using termite-associated nematodes as a model system for phoretic/parasitic organisms which have high habitat specificity and a potential handle (their termite host species) for re-isolation attempts. Methodology: For