revealing the hyperdiverse mite fauna of subarctic canada through dna barcoding揭示了hyperdiverse螨动物靠近北极的加拿大通过dna条码技术.pdfVIP

Revealing the Hyperdiverse Mite Fauna of Subarctic Canada through DNA Barcoding 1 2 1 Monica R. Young *, Valerie M. Behan-Pelletier , Paul D. N. Hebert 1 Biodiversity Institute of Ontario and Department of Integrative Biology, University of Guelph, Guelph, Ontario, Canada, 2 Agriculture and Agri-Food Canada, Ottawa, Ontario, Canada Abstract Although mites are one of the most abundant and diverse groups of arthropods, they are rarely targeted for detailed biodiversity surveys due to taxonomic constraints. We address this gap through DNA barcoding, evaluating acarine diversity at Churchill, Manitoba, a site on the tundra-taiga transition. Barcode analysis of 6279 specimens revealed nearly 900 presumptive species of mites with high species turnover between substrates and between forested and non-forested sites. Accumulation curves have not reached an asymptote for any of the three mite orders investigated, and estimates suggest that more than 1200 species of Acari occur at this locality. The coupling of DNA barcode results with taxonomic assignments revealed that Trombidiformes compose 49% of the fauna, a larger fraction than expected based on prior studies. This investigation demonstrates the efficacy of DNA barcoding in facilitating biodiversity assessments of hyperdiverse taxa. Citation: Young MR, Behan-Pelletier VM, Hebert PDN (2012) Revealing the Hyperdiverse Mite Fauna of Subarctic Canada through DNA Barcoding. PLoS ONE 7(11): e48755. doi:10.1371/journal.pone.0048755 Editor: Brock Fenton, University of Western Ontario, Canada Received August 18, 2012; Accepted October 4, 2012; Published November 2, 2012 Copyright: 2012 Young et al. This is an open-access article distributed under the terms of the Creative Commons