role of structure and glycosylation of adsorbed protein films in biolubrication结构和吸附蛋白质的糖基化作用在biolubrication电影.pdfVIP

Role of Structure and Glycosylation of Adsorbed Protein Films in Biolubrication 1 1 2 2 1 Deepak H. Veeregowda , Henk J. Busscher , Arjan Vissink , Derk-Jan Jager , Prashant K. Sharma *, Henny C. van der Mei1 1 Department of Biomedical Engineering, University Medical Center Groningen and University of Groningen, Groningen, The Netherlands, 2 Department of Oral Maxillofacial Surgery, University Medical Center Groningen and University of Groningen, Groningen, The Netherlands Abstract Water forms the basis of lubrication in the human body, but is unable to provide sufficient lubrication without additives. The importance of biolubrication becomes evident upon aging and disease, particularly under conditions that affect secretion or composition of body fluids. Insufficient biolubrication, may impede proper speech, mastication and swallowing, underlie excessive friction and wear of articulating cartilage surfaces in hips and knees, cause vaginal dryness, and result in dry, irritated eyes. Currently, our understanding of biolubrication is insufficient to design effective therapeutics to restore biolubrication. Aim of this study was to establish the role of structure and glycosylation of adsorbed protein films in biolubrication, taking the oral cavity as a model and making use of its dynamics with daily perturbations due to different glandular secretions, speech, drinking and eating, and tooth brushing. Using different surface analytical techniques (a quartz crystal microbalance with dissipation monitoring, colloidal probe atomic force microscopy, contact angle measurements and X-ray photo-electron spectroscopy), we demonstrated that adsorb