roles of brca2 (fancd1) in oocyte nuclear architecture, gametogenesis, gonad tumors, and genome stability in zebrafishbrca2的角色(fancd1)在卵母细胞的核体系结构中,配子发育、性腺肿瘤,和斑马鱼的基因组稳定.pdfVIP

Roles of brca2 (fancd1) in Oocyte Nuclear Architecture, Gametogenesis, Gonad Tumors, and Genome Stability in Zebrafish ´ ´1 1 1 ˜ 1¤ 1 Adriana Rodrıguez-Marı , Catherine Wilson , Tom A. Titus , Cristian Canestro , Ruth A. BreMiller , Yi- 1 2 3 3 1 1 4 Lin Yan , Indrajit Nanda , Adam Johnston , John P. Kanki , Erin M. Gray , Xinjun He , Jan Spitsbergen , 2 1 Detlev Schindler , John H. Postlethwait * ¨ ¨ 1 Institute of Neuroscience, University of Oregon, Eugene, Oregon, United States of America, 2 Institute of Human Genetics, Biocenter, University of Wurzburg, Wurzburg, Germany, 3 Dana Farber Cancer Institute, Boston, Massachusetts, United States of America, 4 Marine and Freshwater Biomedical Sciences Center, Oregon State University, Corvallis, Oregon, United States of America Abstract Mild mutations in BRCA2 (FANCD1) cause Fanconi anemia (FA) when homozygous, while severe mutations cause common cancers including breast, ovarian, and prostate cancers when heterozygous. Here we report a zebrafish brca2 insertional mutant that shares phenotypes with human patients and identifies a novel brca2 function in oogenesis. Experiments showed that mutant embryos and mutant cells in culture experienced genome instability, as do cells in FA patients. In wild- typ