rnai screen in drosophila cells reveals the involvement of the tom complex in chlamydia infectionrnai屏幕在果蝇细胞中揭示了汤姆的参与复杂的衣原体感染.pdfVIP

RNAi Screen in Drosophila Cells Reveals the Involvement of the Tom Complex in Chlamydia Infection ´ 1* 2,3 4,5 ´ 1 Isabelle Derre , Marc Pypaert , Alice Dautry-Varsat , Herve Agaisse 1 Section of Microbial Pathogenesis, Yale University School of Medicine, New Haven Connecticut, United States of America, 2 Department of Cell Biology, Yale University School of Medicine, New Haven, Connecticut, United States of America, 3 Center for Cell and Molecular Imaging, Yale University School of Medicine, New Haven, ´ Connecticut, United States of America, 4 Unite de Biologie des Interactions Cellulaires, Institut Pasteur, Paris, France, 5 CNRS URA 2582, Paris, France Chlamydia spp. are intracellular obligate bacterial pathogens that infect a wide range of host cells. Here, we show that C. caviae enters, replicates, and performs a complete developmental cycle in Drosophila SL2 cells. Using this model system, we have performed a genome-wide RNA interference screen and identified 54 factors that, when depleted, inhibit C. caviae infection. By testing the effect of each candidate’s knock down on L. monocytogenes infection, we have identified 31 candidates presumably specific of C. caviae infection. We found factors expected to have an effect on Chlamydia infection, such as heparansulfate glycosaminoglycans and actin and microtubule remodeling factors. We also identified factors that were not previously described as involved in Chlamydia infection. For instance, we identified members of the Tim-Tom complex, a multiprotein complex involved in the recognition and import of nuclear-encoded proteins to the mitochondria, as required for C. caviae infection of Drosophila cells. Finally, we confirmed that depletion