rnase 7 contributes to the cutaneous defense against enterococcus faecium核糖核酸酶7有助于皮肤的防御肠球菌都有效.pdfVIP

RNase 7 Contributes to the Cutaneous Defense against Enterococcus faecium ¨ 1. 1. ¨ 1 2 ¨ 1 ¨ Bente Koten , Maren Simanski , Regine Glaser , Rainer Podschun , Jens-Michael Schroder , Jurgen Harder1* 1 Department of Dermatology, University Hospital Schleswig-Holstein, Campus Kiel, Kiel, Germany, 2 Institute for Infection Medicine, University Hospital Schleswig- Holstein, Campus Kiel, Kiel, Germany Abstract Background: Human skin is able to mount a fast response against invading microorganisms by the release of antimicrobial proteins such as the ribonuclease RNase 7. Because RNase 7 exhibits high activity against Enterococcus faecium the aim of this study was to further explore the role of RNase 7 in the cutaneous innate defense system against E. faecium. Methodology/Principal Findings: Absolute quantification using real-time PCR and ELISA revealed that primary keratinocytes expressed high levels of RNase 7. Immunohistochemistry showed RNase 7 expression in all epidermal layers of the skin with an intensification in the upper more differentiated layers. Furthermore, RNase 7 was secreted by keratinocytes in vitro and in vivo in a site-dependent way. RNase 7 was still active against E. faecium at low pH (5.5) or high NaCl (150 mM) concentration and the bactericidal activity of RNase 7 against E. faecium required no ribonuclease activity as shown by recombinant RNase 7 lacking enzymatic activity. To further explore the role of RNase 7 in cutaneous defense against E. faecium, we investigated whether RNase 7 contributes to the E. faecium killing activity of skin extracts derived from stratum corneum. Treatment of the sk