rna-seq analysis of the sclerotinia homoeocarpa – creeping bentgrass pathosystemrna-seq菌核病的分析homoeocarpau2014u2014爬bentgrass pathosystem.pdfVIP

RNA-Seq Analysis of the Sclerotinia homoeocarpa – Creeping Bentgrass Pathosystem 1 . 1. 2 3 Angela M. Orshinsky * , Jinnan Hu , Stephen O. Opiyo , Venu Reddyvari-Channarayappa , Thomas K. Mitchell1, Michael J. Boehm 1 1 Department of Plant Pathology, The Ohio State University, Columbus, Ohio, United States of America, 2 Molecular and Cellular Imaging Center-Columbus. The Ohio State University, Columbus, Ohio, United States of America, 3 Dale Bumpers National Rice Research Center, Stuttgart, Arizona, United States of America Abstract Sclerotinia homoeocarpa causes dollar spot disease, the predominate disease on highly-maintained turfgrass. Currently, there are major gaps in our understanding of the molecular interactions between S. homoeocarpa and creeping bentgrass. In this study, 454 sequencing technology was used in the de novo assembly of S. homoeocarpa and creeping bentgrass transcriptomes. Transcript sequence data obtained using Illumina’s first generation sequencing-by-synthesis (SBS) were mapped to the transcriptome assemblies to estimate transcript representation in different SBS libraries. SBS libraries included a S. homoeocarpa culture control, a creeping bentgrass uninoculated control, and a library for creeping bentgrass inoculated with S. homoeocarpa and incubated for 96 h. A Fisher’s exact test was performed to determine transcripts that were significantly different during creeping bentgrass infection with S. homoeocarpa. Fungal transcripts of interest included glycosyl hydrolases, proteases, and ABC transporters. Of particular interest were the large number of glycosyl hydrolase transcripts that target a wide range of plant cell wall compounds, corroborati