rna-seq analysis reveals different dynamics of differentiation of human dermis- and adipose-derived stromal stem cellsrna-seq分析揭示了不同动力学的人类皮肤和脂肪基质干细胞的分化.pdfVIP

RNA-Seq Analysis Reveals Different Dynamics of Differentiation of Human Dermis- and Adipose-Derived Stromal Stem Cells ¨ ¨ 1,2 3 3 3 1,4 Kersti Jaager *, Saiful Islam , Pawel Zajac , Sten Linnarsson , Toomas Neuman 1 Institute of Gene Technology, Tallinn University of Technology, Tallinn, Estonia, 2 Cellin Technologies LLC, Tallinn, Estonia, 3 Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden, 4 Protobios LLC, Tallinn, Estonia Abstract Background: Tissue regeneration and recovery in the adult body depends on self-renewal and differentiation of stem and progenitor cells. Mesenchymal stem cells (MSCs) that have the ability to differentiate into various cell types, have been isolated from the stromal fraction of virtually all tissues. However, little is known about the true identity of MSCs. MSC populations exhibit great tissue-, location- and patient-specific variation in gene expression and are heterogeneous in cell composition. Methodology/Principal Findings: Our aim was to analyze the dynamics of differentiation of two closely related stromal cell types, adipose tissue-derived MSCs (AdMSCs) and dermal fibroblasts (FBs) along adipogenic, osteogenic and chondrogenic lineages using multiplex RNA-seq technology. We found that undifferentiated donor-matched AdMSCs and FBs are distinct populations that stay different upon differentiation into adipocytes, osteoblasts and chondrocytes. The changes in lineage- specific gene expression occur early in differentiation and persist over time in both AdMSCs and FBs. Further, AdMSCs and FBs exhibit similar dynamics of adipogenic and osteogenic differentiation but different dynamics of chondrogeni