rna interference in schistosoma mansoni schistosomula selectivity, sensitivity and operation for larger-scale screeningrna干扰在曼氏裂体吸虫schistosomula选择性、灵敏度和操作大规模筛选.pdfVIP

RNA Interference in Schistosoma mansoni Schistosomula: Selectivity, Sensitivity and Operation for Larger-Scale Screening ˇ ˇ ´ 1.¤a ˇ ´ 1.¤b 2 1¤c 3 1¤d Sasa Stefanic , Jan Dvorak , Martin Horn , Simon Braschi , Daniel Sojka , Debbie S. Ruelas , 1 1 1 1 1 Brian Suzuki , Kee-Chong Lim , Stephanie D. Hopkins , James H. McKerrow , Conor R. Caffrey * 1 Sandler Center for Drug Discovery, California Institute for Quantitative Biosciences (QB3), University of California San Francisco, San Francisco, California, United States of America, 2 Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, Praha, Czech Republic, 3 Institute of Parasitology, Biology Centre, ˇ ´ ˇ Academy of Sciences of the Czech Republic, Ceske Budejovice, Czech Republic Abstract Background: The possible emergence of resistance to the only available drug for schistosomiasis spurs drug discovery that has been recently incentivized by the availability of improved transcriptome and genome sequence information. Transient RNAi has emerged as a straightforward and important technique to interrogate that information through decreased or loss of gene function and identify potential drug targets. To date, RNAi studies in schistosome stages infecting humans have focused on single (or up to 3) genes of interest. Therefore, in the context of standardizing large