rssab signaling coordinates early development of surface multicellularity in serratia marcescensrssab信号坐标的早期发展表面粘质沙雷氏菌的多细胞生物.pdfVIP

RssAB Signaling Coordinates Early Development of Surface Multicellularity in Serratia marcescens 1 2 3 1 1 4 Yu-Huan Tsai , Jun-Rong Wei , Chuan-Sheng Lin , Po-Han Chen , Stella Huang , Yu-Ching Lin , Chia- 4 5 4 Fong Wei , Chia-Chen Lu , Hsin-Chih Lai * 1 Department of Clinical Laboratory Sciences and Medical Biotechnology, National Taiwan University College of Medicine, Taipei, Taiwan, Republic of China, 2 Department of Immunology and Infectious Diseases, Harvard School of Public Health, Boston, Massachusetts, United States of America, 3 Department of Biochemistry and Molecular Biology, Chang Gung University, Kweishan, Taoyuan, Taiwan, Republic of China, 4 Department of Medical Biotechnology and Laboratory Science, and Research Center for Pathogenic Bacteria, Chang Gung University, Kwei-Shan, Tao-Yuan, Taiwan, Republic of China, 5 Department of Respiratory Therapy, Fu Jen Catholic University, Sinjhuang, Taipei, Taiwan, Republic of China Abstract Bacteria can coordinate several multicellular behaviors in response to environmental changes. Among these, swarming and biofilm formation have attracted significant attention for their correlation with bacterial pathogenicity. However, little is known about when and where the signaling occurs to trigger either swarming or biofilm formation. We have previously identified an RssAB two-component system involved in the regulation of swarming motility and biofilm formation in Serratia marcescens. Here we monitored the RssAB signaling status within single cells by tracing the location of the translational fusion protein EGFP-RssB following development of swarming or biofilm formation. RssAB signaling is specifically activated before surface migration in s