salmonella pathogenicity island 2 is expressed prior to penetrating the intestine沙门氏菌致病性岛2表示之前渗透小肠.pdfVIP

Salmonella Pathogenicity Island 2 Is Expressed Prior to Penetrating the Intestine 1[ 2[ 1 1 1 1* Nat F. Brown , Bruce A. Vallance , Brian K. Coombes , Yanet Valdez , Bryan A. Coburn , B. Brett Finlay 1 Michael Smith Laboratories, University of British Columbia, Vancouver, British Columbia, Canada, 2 Division of Gastroenterology, BC Children’s Hospital, Vancouver, British Columbia, Canada Salmonella enterica serovar Typhimurium is a facultative intracellular pathogen that causes disease in mice that resembles human typhoid. Typhoid pathogenesis consists of distinct phases in the intestine and a subsequent systemic phase in which bacteria replicate in macrophages of the liver and spleen. The type III secretion system encoded by Salmonella pathogenicity island 2 (SPI-2) is a major virulence factor contributing to the systemic phase of typhoid pathogenesis. Understanding how pathogens regulate virulence mechanisms in response to the environment, including different host tissues, is key to our understanding of pathogenesis. A recombinase-based in vivo expression technology system was developed to assess SPI-2 expression during murine typhoid. SPI-2 expression was detectable at very early times in bacteria that were resident in the lumen of the ileum and was independent of active bacterial invasion of the epithelium. We also provide direct evidence for the regulation of SPI-2 by the Salmonella transcription factors ompR and ssrB in vivo. Together these results demonstrate that SPI-2 expression precedes penetration of the intestinal epithelium. This induction of expression precedes any documented SPI-2-dependent phases of typhoid and may be involved in preparing Salmonella to successfully resist the antimicrobial envir