sampling site matters when counting lymphocyte subpopulations采样站点问题时淋巴细胞计数的亚种.pdfVIP

Sampling Site Matters When Counting Lymphocyte Subpopulations 1 2,3 1,4 5 6,7 Benson Ogunjimi *, Dieter Peeters , Niel Hens , Ronald Malfait , Viggo Van Tendeloo , Pierre Van Damme8, Philippe Beutels1,9., Evelien Smits6,7. 1 Centre for Health Economics Research and Modeling Infectious Diseases, Vaccine and Infectious Disease Institute, University of Antwerp, Antwerp, Belgium, 2 Translational Cancer Research Unit, Oncology Center, GZA Hospitals Sint-Augustinus, Antwerp, Belgium, 3 Department of Medical Oncology, University of Antwerp and Antwerp University Hospital, Antwerp, Belgium, 4 Interuniversity Institute for Biostatistics and Statistical Bioinformatics, Hasselt University, Hasselt, Belgium, 5 Department of Laboratory Medicine, Antwerp University Hospital, Antwerp, Belgium, 6 Laboratory of Experimental Hematology, Vaccine and Infectious Disease Institute, University of Antwerp, Antwerp, Belgium, 7 Center for Cell Therapy and Regenerative Medicine, Antwerp University Hospital, Antwerp, Belgium, 8 Centre for the Evaluation of Vaccination, Vaccine and Infectious Disease Institute, University of Antwerp, Antwerp, Belgium, 9 School of Public Health and Community Medicine, University of New South Wales, Sydney, Australia Abstract Clinical and scientific work routinely relies on antecubital venipunctures for hematological, immunological or other analyses on blood. This study tested the hypothesis that antecubital veins can be considered to be a good proxy for other sampling sites. Using a hematocytometer and a flow cytometer, we analyzed the cell counts from samples coming from the radial artery, the dorsal hand veins and the antecubital veins from 18 volunteers. Most surprisingly, we identified the greate