role of gp82 in the selective binding to gastric mucin during oral infection with trypanosoma cruzi角色的gp82选择性结合胃粘蛋白在口腔感染鲁兹锥体.pdfVIP

Role of GP82 in the Selective Binding to Gastric Mucin during Oral Infection with Trypanosoma cruzi 1 1 1 2 1,3 Daniela I. Staquicini , Rafael M. Martins , Silene Macedo , Gisela R. S. Sasso , Vanessa D. Atayde , 4 1 Maria A. Juliano , Nobuko Yoshida * ˜ ˜ 1 Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de Sao Paulo, Sao Paulo, Brasil, 2 Departamento de Morfologia, Universidade Federal ˜ ˜ de Sao Paulo, Sao Paulo, Brasil, 3 Department of Internal Medicine and Cell Biology, Yale University Medical School, New Haven, Connecticut, United States of America, ´ ˜ ˜ 4 Departamento de Biofısica, Universidade Federal de Sao Paulo, Sao Paulo, Brasil Abstract Oral infection by Trypanosoma cruzi has been the primary cause of recent outbreaks of acute Chagas’ diseases. This route of infection may involve selective binding of the metacyclic trypomastigote surface molecule gp82 to gastric mucin as a first step towards invasion of the gastric mucosal epithelium and subsequent systemic infection. Here we addressed that question by performing in vitro and in vivo experiments. A recombinant protein containing the complete gp82 sequence (J18), a construct lacking the gp82 central domain (J18*), and 20-mer synthetic peptides based on the gp82 central domain, were used for gastric mucin binding and HeLa cell invasion assays, or for in