role of gd3-clipr-59 association in lymphoblastoid t cell apoptosis triggered by cd95fasgd3-clipr-59协会在lymphoblastoid t细胞凋亡中的作用由cd95fas触发.pdfVIP

Role of GD3-CLIPR-59 Association in Lymphoblastoid T Cell Apoptosis Triggered by CD95/Fas 1,2 3 1 4 4 Maurizio Sorice *, Paola Matarrese , Valeria Manganelli , Antonella Tinari , Anna Maria Giammarioli , 1,2 1 1,2. 3. Vincenzo Mattei , Roberta Misasi , Tina Garofalo , Walter Malorni 1 Department of Experimental Medicine, ‘‘Sapienza’’ University of Rome, Rome, Italy, 2 Laboratory of Experimental Medicine and Environmental Pathology, ‘‘Sapienza’’ ` University, Rieti, Italy, 3 Department of Drug Research and Evaluation, Section of Cell Aging and Degeneration, Istituto Superiore di Sanita, Rome, Italy, 4 Department of ` Technology and Health, Section of Ultrastructural Infectious Pathology, Istituto Superiore di Sanita, Rome, Italy Abstract We previously found that a directional movement of the raft component GD3 towards mitochondria, by its association with microtubules, was mandatory to late apoptogenic events triggered by CD95/Fas. Since CLIPR-59, CLIP-170-related protein, has recently been identified as a microtubule binding protein associated with lipid rafts, we analyzed the role of GD3-CLIPR- 59 association in lymphoblastoid T cell apoptosis triggered by CD95/Fas. To test whether CLIPR-59 could play a role at the raft-microtubule junction, we performed a series of experiments by using immunoelectron microscopy, static or flow cytometry and biochem