scabies mite peritrophins are potential targets of human host innate immunity疥疮螨peritrophins是人类宿主先天免疫的潜在目标.pdfVIP

Scabies Mite Peritrophins Are Potential Targets of Human Host Innate Immunity 1. 1. 2 1 1 Angela Mika , Priscilla Goh , Deborah C. Holt , Dave J. Kemp , Katja Fischer * 1 Queensland Institute of Medical Research and Australian Centre for International and Tropical Health and Nutrition, University of Queensland, Brisbane, Queensland, Australia, 2 Menzies School of Health Research, Charles Darwin University, Darwin, Northern Territory, Australia Abstract Background: Pruritic scabies lesions caused by Sarcoptes scabiei burrowing in the stratum corneum of human skin facilitate opportunistic bacterial infections. Emerging resistance to current therapeutics emphasizes the need to identify novel targets for protective intervention. We have characterized several protein families located in the mite gut as crucial factors for host- parasite interactions. Among these multiple proteins inhibit human complement, presumably to avoid complement- mediated damage of gut epithelial cells. Peritrophins are major components of the peritrophic matrix often found in the gut of arthropods. We hypothesized that a peritrophin, if abundant in the scabies mite gut, could be an activator of complement. Methodology/Principal Findings: A novel full length scabies mite peritrophin (SsPTP1) was identified in a cDNA library from scabies mites. The amino acid sequence revealed four putative chitin binding domains (CBD). Recombinant expression of one CBD of the highly repetitive SsPTP1 sequence as TSP-hexaHis-fusion protein resulted in soluble protein, which demonstrated chitin binding activity in affinity chromatography assays. Antibodies against a recombinant SsPTP1 fragment