self-assembling complexes of quantum dots and scfv antibodies for cancer cell targeting and imaging自组装量子点和scfv抗体的复合物对癌症细胞定位和成像.pdfVIP

Self-Assembling Complexes of Quantum Dots and scFv Antibodies for Cancer Cell Targeting and Imaging 1,2 1 1 1,3 Tatiana A. Zdobnova *, Oleg A. Stremovskiy , Ekaterina N. Lebedenko , Sergey M. Deyev 1 Institute of Bioorganic Chemistry of the Russian Academy of Sciences, Moscow, Russia, 2 Department of Biology, Lobachevsky State University Nizhny Novgorod, Nizhny Novgorod, Russia, 3 Research Institute of Applied and Fundamental Medicine, Nizhny Novgorod State Medical Academy, Nizhny Novgorod, Russia Abstract Semiconductor quantum dots represent a novel class of fluorophores with unique physical and chemical properties which could enable a remarkable broadening of the current applications of fluorescent imaging and optical diagnostics. Complexes of quantum dots and antibodies are promising visualising agents for fluorescent detection of selective biomarkers overexpressed in tumor tissues. Here we describe the construction of self-assembling fluorescent complexes of quantum dots and anti-HER1 or anti-HER2/neu scFv antibodies and their interactions with cultured tumor cells. A binding strategy based on a very specific non-covalent interaction between two proteins, barnase and barstar, was used to connect quantum dots and the targeting antibodies. Such a strategy allows combining the targeting and visualization functions simply by varying the corresponding modules of the fluorescent complex. Citation: Zdobnova TA, Stremovskiy OA, Lebedenko EN, Deyev SM (2012) Self-Assembling Complexes of Quantum Dots and scFv Antibodies for Cancer Cell Targeting and Imaging. PLoS ONE 7(10): e48248. doi:10.1371/journal.pone.0048248 Editor: Vladimir V. Kalinichenko, Cincinnat